Agilent Technologies 1100 Series Manuale delle Istruzioni Scaricare il pdf (Pagina 34)

Optimizing Performance

Solvent Information

Always filter solvents through 0.4 µm filters, small particles can permanently

block the capillaries and valves. Avoid the use of the following

steel-corrosive solvents:

• Solutions of alkali halides and their respective acids (for example, lithium

iodide, potassium chloride, and so on).

• High concentrations of inorganic acids like sulfuric acid, especially at

higher temperatures (replace, if your chromatography method allows, by

phosphoric acid or phosphate buffer which are less corrosive against

stainless steel).

• Halogenated solvents or mixtures which form radicals and/or acids, for

example:

2CHCl

+ O

→ 2COCl

+ 2HCl

This reaction, in which stainless steel probably acts as a catalyst, occurs

quickly with dried chloroform if the drying process removes the

stabilizing alcohol.

• Chromatographic grade ethers, which can contain peroxides (for example,

THF, dioxane, di-isopropylether) such ethers should be filtered through

dry aluminium oxide which adsorbs the peroxides.

• Mixtures of carbon tetrachloride with 2-propanol or THF dissolve stainless

steel.

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Agilent Technologies 1100 Series Manuale delle Istruzioni Pagina 34