Agilent Technologies DP235 Guida Utente Scaricare il pdf (Pagina 162)

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Contents ▲ 162 ▼ Index

Generating Histograms

2100 expert counts the events, sorts them and displays them according to their

fluorescence intensity in histograms. For each color measured, a histogram displays the

number of events related to the fluorescence intensity. A large number of events with a

high fluorescence value means that a large number of cells containing the fluorescence

dye were detected.

In the following illustration, cells which fluoresce in both colors are highlighted.

As a cell passes through the detector, its blue and red fluorescence values

are measured and the count is increased for both channels at the

appropriate intensity.

Illustrates a cell that

fluoresces in blue and

red. The height of the

bars is related to the

number of cells with this

fluorescence value.

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Agilent Technologies DP235 Guida Utente Pagina 162

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