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Contents ▲ 97 ▼ Index

Data Analysis: RNA and Cy5-Labeled Nucleic Acids

The data analysis process for RNA and the Cy5-labeled nucleic acids assays consists of

the following steps:

1. Raw data is read and stored by the system for all of the individual samples.

2. The data is filtered and the resulting electropherograms of all samples are plotted. You

can change the settings of the data analysis after the run and reanalyze your data.

3. Fragments are identified for all samples and tabulated by peak ID. You can change the

settings of the peak find algorithm for any or all samples and reanalyze the data.

4. An RNA ladder (containing a mixture of RNA of known concentration) is run first (see

the electropherogram below). The concentrations and sizes of the individual base pairs

are preset in the assay and cannot be changed.

Electropherogram of RNA 6000 Ladder (Ambion, Inc. cat. no. 7152)

1 2 ... 92 93 94 95 96 97 98 99 100 101 102 ... 601 602

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Agilent Technologies DP235 Guida Utente Pagina 97

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