Agilent Technologies DP235 Guida Utente Scaricare il pdf (Pagina 206)

Contents ▲ 206 ▼ Index

The following image shows two histograms with a gating direction from blue to red (left

to right) of an apoptosis assay. The blue histogram shows calcein fluorescence, which

indicates living or dead cells (high fluorescence value means living cells). The red

histogram shows the subpopulation of living cells with annexin V fluorescence indicating

apoptosis (high fluorescence value means the cell is apoptotic). As a result you can see a

subset of living, apoptotic cells.

Fluorescence

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Agilent Technologies DP235 Guida Utente Pagina 206