Agilent Technologies DP235 Guida Utente Scaricare il pdf (Pagina 232)

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Contents ▲ 232 ▼ Index

When using the calcein marker in the blue histogram for gating, only living cells are

considered for building the histogram of the red dye. High red fluorescence values

indicate living cells with bound antibodies, low red fluorescence values living cells

without bound antibodies. See the following example.

The values are displayed in the result table below the red histogram:

Low fluorescence values

indicate low binding of

labeled antibodies

High fluorescence

values indicate binding

of labeled antibodies

Percentage of all cells with

high red fluorescence

selected by the red marker

Percentage of the living cells

Amount of living cells with high red fluorescence in relation to the amount of living cells

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Agilent Technologies DP235 Guida Utente Pagina 232

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