Agilent Technologies DP235 Guida Utente Scaricare il pdf (Pagina 68)

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Contents ▲ 68 ▼ Index

• Protect dye and gel-dye mix from light. Remove light covers only when pipetting. Dye

decomposes when exposed to light.

• Use a new syringe and electrode cleaner with each new LabChip kit.

• Do not touch the Agilent 2100 bioanalyzer during a chip run and never place it on a

vibrating ground.

• Keep all reagents and reagent mixes refrigerated at 4 °C when not in use.

• Allow all reagents and samples to equilibrate to room temperature for 30 minutes

before use.

• Use loaded chips within 5 minutes. Reagents might evaporate, leading to poor results.

RNA Assays:

• Always wear gloves when handling RNA, and use RNase-free tips, microfuge tubes and

water.

• It is recommended to denature all RNA samples and RNA ladder by heat before use

(70 °C, 2 minutes).

• Always vortex the dye concentrate for 10 seconds before preparing the gel-dye mix.

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Agilent Technologies DP235 Guida Utente Pagina 68

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