Agilent Technologies DP235 Guida Utente Pagina 236
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Contents ▲ 236 ▼ Index
Histogram evaluation
The two histograms displaying the results of the assay are related to calcein (blue
fluorescence) and annexin V (red fluorescence). High fluorescence values in the blue
histogram indicate living cells, low values correspond to dead cells. See the following
image as an example.
The values are displayed in the result table, each histogram has its own table:
Low fluorescence value
indicates dead cells
High fluorescence value
indicates living cells
All measured events
All events in relation to the
blue marker (here calcein)
Living cells in relation to all measured cells (high calcein fluorescence)
- Agilent 2100 Bioanalyzer 1
- 2100 Expert User’s Guide 1
- Contents ▲ 2 ▼ Index 2
- Contents 3
- Contents ▲ 4 ▼ Index 4
- Contents ▲ 5 ▼ Index 5
- About this Manual 6
- Contents ▲ 7 ▼ Index 7
- In this Manual 7
- Contents ▲ 8 ▼ Index 8
- Contents ▲ 9 ▼ Index 9
- Related Documents 9
- Contents ▲ 10 ▼ Index 10
- Contents ▲ 11 ▼ Index 11
- What’s New in 2100 Expert 11
- Contents ▲ 12 ▼ Index 12
- Contents ▲ 13 ▼ Index 13
- How to Use this Manual 13
- ▲ Displays the previous page 13
- ▼ Displays the next page 13
- Contents ▲ 14 ▼ Index 14
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- Quick Start 17
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- Looking at 2100 Expert 31
- Contents ▲ 32 ▼ Index 32
- Starting 2100 Expert 32
- Contents ▲ 33 ▼ Index 33
- 2100 Expert Work Area 33
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- Running a Demo Assay 41
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- Closing 2100 Expert 45
- Contents ▲ 46 ▼ Index 46
- Cytometric Assays 47
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- Result Flagging 145
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- 2100 Expert Data Overview 246
- Contents ▲ 247 ▼ Index 247
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- Handling Assays 249
- Contents ▲ 250 ▼ Index 250
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- Handling Chip Data 253
- Contents ▲ 254 ▼ Index 254
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- Importing Data 256
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- Exporting Data 263
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- Printing Reports 275
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- Configuring Tables 282
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- Reading the Log Books 287
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- Configuring 2100 Expert 293
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- How to Specify Graph Settings 298
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- Performing Qualifications 312
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