Agilent Technologies DP235 Guida Utente Scaricare il pdf (Pagina 95)

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Contents ▲ 95 ▼ Index

6. Two DNA fragments are run with each of the samples, bracketing the DNA sizing range.

The “lower marker” and “upper marker” are internal standards used to align the ladder

data with data from the sample wells. The figure below shows an example of assigned

marker peaks in a sample well.

NOTE

The software performs alignment by default. Turning automatic data analysis off (see

“Electropherogram Menu” on page 345) suspends data analysis until you turn it on

again.

7. The standard curve, in conjunction with the markers, is used to calculate DNA fragment

sizes for each sample from the migration times measured.

Lower marker

Upper marker

1 2 ... 90 91 92 93 94 95 96 97 98 99 100 ... 601 602

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Agilent Technologies DP235 Guida Utente Pagina 95

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